Identification of bacterial glycosidases in rat cecal contents

  title={Identification of bacterial glycosidases in rat cecal contents},
  author={Dr. Roberto Prizont and Nancy Konigsberg},
  journal={Digestive Diseases and Sciences},
Cecal contents of conventional and germfree rats were examined for glycosidases which may have a role in degrading glycoprotein oligosaccharides. Utilizing p-nitrophenylglycosides as substrates, we identified glycosidases in bacteria-free supernatants from cecal contents which act on β-linkages. These cecal glycosidases appear to be of bacterial origin since: (1) direct comparisons of the enzymes in similar contents from germfree rats showed negligible activities; (2) most of the glycosidase… CONTINUE READING


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