"Transcription physiology" of pigment formation in melanocytes: central role of MITF.
Fig. 1. Screening for natural anti-melanogenic products. (A) B16F1 Cells purchased from medium supplemented with 10% (v/v) fetal bovine serum with 0.1% gentamycin (Ch 1 10 cells/well (6-well plate) and were incubated overnight and then treated with vari taken using a digital camera. (B) The synthesized melanin pellet with pretreatment of n described previously . (C) Cell pellet images after 72 h treatment with FL. (D) Tyrosinas free system. (E) Compared with the control, mRNA levels of tyrosinase, MITF, and MC1R in primers were designed to amplify murine tyrosinase (forward: 50-GTCGTCACCCTGAA ACGACAACTCTGGATCTCAC-30 , reverse: 50-CATCAGGATGTCTTCCAAGT-30); MC1R (forw and the housekeeping gene GAPDH. Data are expressed as a percentage of the control a **p < 0.01, versus control.