Identification of an operator sequence for the Bacillus subtilis gnt operon.

  title={Identification of an operator sequence for the Bacillus subtilis gnt operon.},
  author={Yasutaro Fujita and Yasuhiko Miwa},
  journal={The Journal of biological chemistry},
  volume={264 7},
Expression of the Bacillus subtilis gluconate (gnt) operon is negatively regulated by the gnt repressor which is antagonized by D-gluconate or D-glucono-delta-lactone. The repressor specifically binds to a gnt operator near the gnt promoter. From the results of gel retardation assaying of the gnt repressor and various restriction enzyme digests of a fragment carrying the gnt promoter and the gntR gene, the gnt operator was found to be located within a 141-base pair region containing the gnt… CONTINUE READING
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