Identification of an essential gene responsible for D-Asp incorporation in the Lactococcus lactis peptidoglycan crossbridge.

@article{Veiga2006IdentificationOA,
  title={Identification of an essential gene responsible for D-Asp incorporation in the Lactococcus lactis peptidoglycan crossbridge.},
  author={P. Veiga and Sandra Piquet and Aur{\'e}lie Maisons and Sylviane Furlan and Pascal Courtin and Marie-Pierre Chapot-Chartier and Saulius Kulakauskas},
  journal={Molecular microbiology},
  year={2006},
  volume={62 6},
  pages={1713-24}
}
Bacteria such as Lactococcus lactis have D-aspartate (D-Asp) or its amidated derivative D-asparagine (D-Asn), in their peptidoglycan (PG) interpeptide crossbridge. We performed a subtractive genome analysis to identify L. lactis gene yxbA, orthologues of which being present only in bacteria containing D-amino acids in their PG crossbridge, but absent from those that instead insert L-amino acids or glycine. Inactivation of yxbA required a complementing Streptococcus pneumoniae murMN genes, which… CONTINUE READING