Identification of an endosomal antigen specific to absorptive cells of suckling rat ileum

@article{Wilson1987IdentificationOA,
  title={Identification of an endosomal antigen specific to absorptive cells of suckling rat ileum},
  author={Jean M Wilson and Jennifer A. Whitney and Marian R. Neutra},
  journal={The Journal of Cell Biology},
  year={1987},
  volume={105},
  pages={691 - 703}
}
A membrane fraction enriched in apical endosomal tubules was isolated from absorptive cells of suckling rat ileum and used as an immunogen to generate anti-endosome monoclonal antibodies. By immunofluorescence, one of these antibodies bound exclusively to the region of the apical endocytic complex in ileal absorptive cells, but not to other cell types. Immunoblot analysis showed the antigen as a diffuse 55-61-kD band which was highly enriched in the endosome fraction over whole-cell homogenate… CONTINUE READING
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