Identification of an amino acid determinant of pH regiospecificity in a seed lipoxygenase from Momordica charantia.

@article{Hornung2008IdentificationOA,
  title={Identification of an amino acid determinant of pH regiospecificity in a seed lipoxygenase from Momordica charantia.},
  author={Ellen Hornung and Susan Kunze and Alena Liavonchanka and Grit Zimmermann and Diana Kuehn and Kathrin Fritsche and Andreas Renz and Hartmut K{\"u}hn and Ivo Feussner},
  journal={Phytochemistry},
  year={2008},
  volume={69 16},
  pages={2774-80}
}
Lipoxygenases (LOX) form a heterogeneous family of lipid peroxidizing enzymes, which catalyze specific dioxygenation of polyunsaturated fatty acids. According to their positional specificity of linoleic acid oxygenation plant LOX have been classified into linoleate 9- and linoleate 13-LOX and recent reports identified a critical valine at the active site of 9-LOX. In contrast, more bulky phenylalanine or histidine residues were found at this position in 13-LOX. We have recently cloned a LOX… CONTINUE READING