Identification of an alpha 2-adrenoceptor in human coronary arteries by radioligand binding assay.

@article{Ishikawa1991IdentificationOA,
  title={Identification of an alpha 2-adrenoceptor in human coronary arteries by radioligand binding assay.},
  author={Yumiko Ishikawa and Satoshi Umemura and Kazuaki Uchino and Tomomi Shindou and Gen Yasuda and Kohsuke Minamisawa and Shun-suke Hayashi and Nobuhito Hirawa and Masao Ishii},
  journal={Life sciences},
  year={1991},
  volume={48 26},
  pages={
          2513-8
        }
}
A single high affinity binding site for an alpha 2-adrenoceptor in human coronary arteries was identified by radioligand binding assay. Human coronary arteries were obtained at autopsy within 6 hours of death. A crude membrane solution was incubated with (3H)-rauwolscine at 25 degrees C for 30 min. The binding of (3H)-rauwolscine was rapidly saturable and… CONTINUE READING