Identification of an N-glycosylated region of pig zona pellucida glycoprotein ZPB that is involved in sperm binding.

@article{Yonezawa1997IdentificationOA,
  title={Identification of an N-glycosylated region of pig zona pellucida glycoprotein ZPB that is involved in sperm binding.},
  author={Naoto Yonezawa and Shinichi Mitsui and Katsuyasu Kudo and Minoru Nakano},
  journal={European journal of biochemistry},
  year={1997},
  volume={248 1},
  pages={86-92}
}
ZPB, one of the pig zona pellucida glycoproteins, can be purified after removal of sialylated and/or sulfated N-acetylpolylactosamine from the nonreducing region of its carbohydrate chains by digestion with endo-beta-galactosidase. Among the components produced, only ZPB shows sperm-binding activity after the digestion. Recently, we have shown that N-linked carbohydrate chains of endo-beta-galactosidase-digested ZPB (EbetaG-ZPB) are predominantly involved in sperm binding [Yonezawa, N., Aoki, H… CONTINUE READING