Identification of an Alu retrotransposition event in close proximity to a strong candidate gene for Huntington's disease.

Abstract

Huntington's disease (HD) is a late-onset autosomal dominant neuropsychiatric disorder presenting in mid-adult life with personality disturbance and involuntary movements, cognitive and affective disturbance, and inexorable progression to death. The underlying genetic defect has been mapped to chromosomal band 4p16.3 (refs 2, 3). Analysis of specific recombination events in some families with HD has further refined the location of the HD defect to a 2.2 megabase DNA interval. Using a direct complementary DNA selection strategy we have identified at least seven transcriptional units within the minimal region believed to contain the HD gene. Screening with one of the cDNA clones identified an Alu insertion in genomic DNA from two persons with HD which showed complete cosegregation with the disease in these families but was not found in 1,000 control chromosomes. Two genes including the previously identified alpha-adducin gene and another that encodes for a 12-kilobase transcript, map in close proximity to the Alu insertion site. The 12-kilobase transcript should be regarded as a strong candidate for the HD gene.

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@article{Goldberg1993IdentificationOA, title={Identification of an Alu retrotransposition event in close proximity to a strong candidate gene for Huntington's disease.}, author={Yigal Paul Goldberg and Johanna M Rommens and Susan E. Andrew and Gordon B. Hutchinson and Biing Yuan Lin and Jane L. Theilmann and Rebecca Graham and Martha L. Glaves and Elizabeth M Starr and Hayes W. McDonald}, journal={Nature}, year={1993}, volume={362 6418}, pages={370-3} }