Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate.

@article{Hills2007IdentificationOA,
  title={Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate.},
  author={Robert Hills and Richard A. Mazzarella and Kam F Fok and Min Liu and Olga V Nemirovskiy and Joseph Leone and Marc D. Zack and Elizabeth C Arner and Malini Viswanathan and Aida Abujoub and A. Muruganandam and Daniel J. Sexton and Gary J Bassill and Aaron K. Sato and A M Malfait and Micky D. Tortorella},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 15},
  pages={11101-9}
}
ADAMTS-4 and ADAMTS-5 are aggrecanases responsible for the breakdown of cartilage aggrecan in osteoarthritis. Multiple ADAMTS-4 cleavage sites have been described in several matrix proteins including aggrecan, versican, and brevican, but no concise predictive cleavage motif has been identified for this protease. By screening a 13-mer peptide library with a diversity of 10(8), we have identified the ADAMTS-4 cleavage motif E-(AFVLMY)-X(0,1)-(RK)-X(2,3)-(ST)-(VYIFWMLA), with Glu representing P1… CONTINUE READING