Identification of amino acid residues in the catalytic domain of RNase E essential for survival of Escherichia coli: functional analysis of DNase I subdomain.

@article{Shin2008IdentificationOA,
  title={Identification of amino acid residues in the catalytic domain of RNase E essential for survival of Escherichia coli: functional analysis of DNase I subdomain.},
  author={Eunkyoung Shin and Hayoung Go and J B Yeom and Miae Won and Jeehyeon Bae and Seung Hyun Han and Kook Il Han and Younghoon Lee and Nam-Chul Ha and Christopher J. Moore and Bj{\"o}rn Sohlberg and Stanley N. Cohen and Kangseok Lee},
  journal={Genetics},
  year={2008},
  volume={179 4},
  pages={1871-9}
}
RNase E is an essential Escherichia coli endoribonuclease that plays a major role in the decay and processing of a large fraction of RNAs in the cell. To better understand the molecular mechanisms of RNase E action, we performed a genetic screen for amino acid substitutions in the catalytic domain of the protein (N-Rne) that knock down the ability of RNase E to support survival of E. coli. Comparative phylogenetic analysis of RNase E homologs shows that wild-type residues at these mutated… CONTINUE READING