Identification of amino acid residues essential for reconstitutive activity of subunit IV of the cytochrome bc1 complex from Rhodobacter sphaeroides.

@article{Tso2006IdentificationOA,
  title={Identification of amino acid residues essential for reconstitutive activity of subunit IV of the cytochrome bc1 complex from Rhodobacter sphaeroides.},
  author={S C Tso and Ying Yin and Chang-an Yu and Linda Yu},
  journal={Biochimica et biophysica acta},
  year={2006},
  volume={1757 12},
  pages={1561-7}
}
A region of subunit IV comprising residues 77-85 is identified as essential for interaction with the core complex to restore the bc(1) activity (reconstitutive activity). Recombinant subunit IV mutants with single or multiple alanine substitution at this region were generated and characterized to identify the essential amino acid residues. Residues 81-84… CONTINUE READING