Identification of active site carboxylic residues in Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase by site-directed mutagenesis.

@article{Juncosa1994IdentificationOA,
  title={Identification of active site carboxylic residues in Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase by site-directed mutagenesis.},
  author={Mar{\'i}a Teresa Juncosa and Jean-Luc Pons and Therese Del Dot and Enric Querol and Antoni Planas},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 20},
  pages={14530-5}
}
Active site residues of 1,3-1,4-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.73) from Bacillus licheniformis have been identified by site-directed mutagenesis. Previous work revealed that Glu-134 was essential for enzymatic activity, and it was proposed as the catalytic nucleophile by affinity labeling of the highly homologous Bacillus amyloliquefaciens enzyme. To search for the general acid catalyst, the Asp and Glu residues conserved among the Bacillus isozymes have been mutated to Asn and Gln… CONTINUE READING

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