Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature‐tagged transposon mutagenesis

@article{Camacho1999IdentificationOA,
  title={Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature‐tagged transposon mutagenesis},
  author={Luis R. Camacho and Danielle Ensergueix and Esther Pérez and Brigitte Gicquel and Christophe Guilhot},
  journal={Molecular Microbiology},
  year={1999},
  volume={34}
}
Tuberculosis remains the greatest cause of death worldwide due to a single pathogen. In order to identify the genes required for the pathogenicity of Mycobacterium tuberculosis, a functional genomic approach was developed. A library of signature‐tagged transposon mutants of this bacterium was constructed and screened for those affected in their multiplication within the lungs of mice. From 1927 mutants tested, 16 were attenuated for their virulence. The insertions harboured by the selected… Expand
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