Identification of a specific one amino acid change in recombinant human transglutaminase 2 that regulates its activity and calcium sensitivity.

  title={Identification of a specific one amino acid change in recombinant human transglutaminase 2 that regulates its activity and calcium sensitivity.},
  author={Kajal Kanchan and Elvan Erg{\"u}len and R{\'o}bert Kir{\'a}ly and Zs{\'o}fia Simon-Vecsei and M{\'o}nika Fuxreiter and L{\'a}szl{\'o} F{\'e}s{\"u}s},
  journal={The Biochemical journal},
  volume={455 3},
TG2 (transglutaminase 2) is a calcium-dependent protein cross-linking enzyme which is involved in a variety of cellular processes. The threshold level of calcium needed for endogenous and recombinant TG2 activity has been controversial, the former being more sensitive to calcium than the latter. In the present study we address this question by identifying a single amino acid change from conserved valine to glycine at position 224 in recombinant TG2 compared with the endogenous sequence present… 

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