Identification of a specific effector of the small GTP-binding protein Rap2.

@article{JanoueixLerosey1998IdentificationOA,
  title={Identification of a specific effector of the small GTP-binding protein Rap2.},
  author={Isabelle Janoueix-Lerosey and Evdokia A. Pasheva and M F de Tand and Armand Tavitian and Jean de Gunzburg},
  journal={European journal of biochemistry},
  year={1998},
  volume={252 2},
  pages={290-8}
}
Rap2 is a small GTP-binding protein that belongs to the Ras superfamily and whose function is still unknown. To elucidate Rap2 function, we searched for potential effectors by screening a mouse brain cDNA library in a yeast two-hybrid system using as a bait a Rap2A protein bearing a mutation of Gly to Val at position 12. This strategy lead to the identification of a protein that interacts specifically with Rap2A complexed with GTP, and requires an intact effector domain of Rap2A for interaction… CONTINUE READING