Identification of a single tryptophan residue as critical for binding activity in a humanized monoclonal antibody against respiratory syncytial virus.

@article{Wei2007IdentificationOA,
  title={Identification of a single tryptophan residue as critical for binding activity in a humanized monoclonal antibody against respiratory syncytial virus.},
  author={Ziping Wei and Jinhua Feng and Hung-yu Lin and Sombabu Mullapudi and Eric Bishop and Guillermo I Tous and Jose Casas-Finet and Fadi Hakki and Robert Strouse and Mark A. Schenerman},
  journal={Analytical chemistry},
  year={2007},
  volume={79 7},
  pages={2797-805}
}
We have identified a single tryptophan (Trp) residue responsible for loss of binding and biological activity upon ultraviolet (UV) light irradiation in MEDI-493, a humanized monoclonal antibody (MAb) against respiratory syncytial virus (RSV). This finding provides a better understanding of structure-function relationship in a 150-kDa protein. Irradiation of MEDI-493 with UV light resulted in spectral changes typical of Trp photoproducts and in a progressive loss of MEDI-493 binding and… CONTINUE READING
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