Identification of a short domain within the non-structural protein NS2 of epizootic haemorrhagic disease virus that is important for single strand RNA-binding activity.

@article{Theron1996IdentificationOA,
  title={Identification of a short domain within the non-structural protein NS2 of epizootic haemorrhagic disease virus that is important for single strand RNA-binding activity.},
  author={Jacques Theron and Henk Huismans and Louis Hendrik Nel},
  journal={The Journal of general virology},
  year={1996},
  volume={77 ( Pt 1)},
  pages={129-37}
}
The role that a conserved amino acid motif, found in the non-structural protein NS2 of orbiviruses, plays in the interaction of this protein with single stranded (ss) RNA was investigated by mutation analysis of the NS2 of epizootic haemorrhagic disease virus. An NS2 mutant in which this motif (amino acids 75 to 83) was deleted was expressed in Spodoptera frugiperda cells by a recombinant baculovirus and found to be unable to bind to poly(U)-Sepharose. The deletion mutant also differed from… CONTINUE READING

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