Identification of a region of the herpes simplex virus single-stranded DNA-binding protein involved in cooperative binding.

@article{Dudas1998IdentificationOA,
  title={Identification of a region of the herpes simplex virus single-stranded DNA-binding protein involved in cooperative binding.},
  author={K C Dudas and William T. Ruyechan},
  journal={Journal of virology},
  year={1998},
  volume={72 1},
  pages={257-65}
}
We have identified a region of the herpes simplex virus major DNA-binding protein (ICP8) which is involved in cooperative binding to single-stranded DNA. This has been accomplished by analysis of ICP8 which was covalently modified by reaction with the extrinsic fluorophore fluorescein-5-maleimide (FM). Reaction conditions which result in the incorporation of 1 mol of FM per mol of ICP8 have been established. The binding properties of the modified protein were analyzed by polyacrylamide gel… CONTINUE READING

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