Identification of a peptide of the guanosine triphosphate binding site within brain glutamate dehydrogenase isoproteins using 8-azidoguanosine triphosphate.

@article{Cho1996IdentificationOA,
  title={Identification of a peptide of the guanosine triphosphate binding site within brain glutamate dehydrogenase isoproteins using 8-azidoguanosine triphosphate.},
  author={S. Cho and J. Ahn and J. Lee and S. Choi},
  journal={Biochemistry},
  year={1996},
  volume={35 44},
  pages={
          13907-13
        }
}
Photoaffinity labeling with [gamma-32P]8N3GTP (8-azidoguanosine triphosphate) was used to identify the guanine binding peptides of the GTT binding site within two types of glutamate dehydrogenase isoproteins (GDH I and GDH II) isolated from bovine brain. 8N3GTP, without photolysis, mimicked the inhibitory properties of GTP on GDH I and GDH II activities. Saturation of photoinsertion of GDH isoproteins revealed an apparent Kd of 8 microM (GDH I) and 24 microM (GDH II) for [gamma-32P]8N3GTP. Ion… Expand
33 Citations
Photoaffinity Labeling of Brain Glutamate Dehydrogenase Isoproteins with an Azido-ADP*
  • S. Cho, H. Yoon
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • 1999
...
1
2
3
4
...