Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution.

@article{Picault2002IdentificationOA,
  title={Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution.},
  author={Nathalie Picault and Luigi Palmieri and Isabella Pisano and Matthew P. P. Hodges and Ferdinando Palmieri},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 27},
  pages={
          24204-11
        }
}
A cDNA from Arabidopsis thaliana and four related cDNAs from Nicotiana tabacum that we have isolated encode hitherto unidentified members of the mitochondrial carrier family. These proteins have been overexpressed in bacteria and reconstituted into phospholipid vesicles. Their transport properties demonstrate that they are orthologs/isoforms of a novel mitochondrial carrier capable of transporting both dicarboxylates (such as malate, oxaloacetate, oxoglutarate, and maleate) and tricarboxylates… CONTINUE READING
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