Identification of a novel human serpin gene; cloning sequencing and expression of leupin

  title={Identification of a novel human serpin gene; cloning sequencing and expression of leupin},
  author={R C Barnes and D. Margaret Worrall},
  journal={FEBS Letters},
Immunoreactivity of recombinant squamous cell carcinoma antigen and leupin/SCCA-2: implications for tumor marker detection.
The confirmation that leupin or SCCA-2 is the more acidic protein and that its expression is significantly elevated in cervical cancer suggests that this gene product may be the more important tumor marker.
Cloning and characterization of hurpin (protease inhibitor 13): A new skin-specific, UV-repressible serine proteinase inhibitor of the ovalbumin serpin family.
In psoriasis, a skin disease characterized by hyperproliferation of keratinocytes and responsive to therapeutic UV irradiation, overexpression of hurpin is noted in psoriatic skin lesions compared to non-lesional skin.
Coagulation, Neurobiology, and Cancer
A novel serpin is discovered, leupin, which is high in sequence identity to SCCA but which contains differences in the reactive loop sequence including the P1 leucine residue, suggesting it may inhibit a different target protease.
SERPINB3 and B4: From biochemistry to biology.
Distribution of the Human Intracellular Serpin Protease Inhibitor 8 in Human Tissues
It is concluded that PI-8 has a unique distribution pattern in human tissues compared to the distribution patterns of other intracellular serpins, and additional studies must be performed to elucidate its physiological role.
Analysis of serpin inhibitory function by mutagenesis of ovalbumin and generation of chimeric ovalbumin/PAI-2 fusion proteins.
Structural differences between the inactive F2 and active F3 include the replacement of helix F and beta sheet strand 3A of ovalbumin with those of PAI-2, suggesting that these features may have a key role in serpin beta-sheet opening and inhibitory function.
SCCA2 inhibits TNF-mediated apoptosis in transfected HeLa cells. The reactive centre loop sequence is essential for this function and TNF-induced cathepsin G is a candidate target.
The ability of SCCA2 to inhibit tumor necrosis factor-alpha (TNF alpha)-induced apoptosis is demonstrated and the RCL amino acids, flanking the predicted cleavage site, were mutated and the resulting S CCA2 lost both the ability to inhibit cathepsin G and to protect stably transfected cells from TNF alpha- induced apoptosis.
Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases.
The results strongly indicate that the serpin body is intrinsically capable of supporting cysteine protease inhibition, and that the complex with a papain-like cystein protease would be expected to be analogous to that seen with serine proteases.
Characterization of Monoclonal Antibodies Directed against Squamous Cell Carcinoma Antigens: Report of the Second TD-10 Workshop
Eight monoclonal antibodies directed against Squamous Cell Carcinoma Antigens (A1 and A2) were collected and evaluated by three working groups, finding that SCC103 is probably the best A2-specific antibody available and one antibody was A1-specific and is related to reference antibody K135.


Cloning and expression of the cDNA for human antithrom, bin III
The complete nucleotide and predicted amino acid sequences of human ATIII and its 32 residue signal peptide are reported, and provide further opportunity to compare the ATIII primary structure with corresponding regions from homologous proteins, alpha 1-antitrypsin and ovalbumin.
The ovalbumin gene family: complete sequence and structure of the Y gene.
The exact location of exons can be derived from the comparison with the ovalbumin gene sequence and from the map previously established by electron microscopy analysis, which shows a significantly high number of base changes within coding sequences are present in clusters, which appear in several cases to be correlated with the occurrence of direct repeats.
Evolutionary relationships among the serpins.
  • C. Marshall
  • Biology
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 1993
The proposed gene tree suggested that as much diversity existed between the plant serpin and mammalian serpin as was present among mammalian serpins and provided further evidence that the architecture of serpin molecules is highly constrained.
Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. An example of cross-class inhibition.
The mechanism and kinetics of this unusual inhibition of a cysteine proteinase by a member of the serpin superfamily previously thought to inhibit serineproteinase only are reported.
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples.
The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight.
Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.
The described cell lines may serve as a more representative model system for studies of regulation of oncodevelopmental genes in gynecological tumors in general and in epidermoid cervical cancer in particular.