Identification of a novel cell attachment domain in the HIV-1 Tat protein and its 90-kDa cell surface binding protein.

@article{Weeks1993IdentificationOA,
  title={Identification of a novel cell attachment domain in the HIV-1 Tat protein and its 90-kDa cell surface binding protein.},
  author={Benjamin S. Weeks and Kemlesh Desai and Paul M. Loewenstein and Mary E. Klotman and Paul E. Klotman and Michael C. Green and Hynda K. Kleinman},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 7},
  pages={5279-84}
}
The HIV-1 transactivator protein Tat is essential for viral gene expression and replication. Tat is taken up by cells and transactivates the HIV-LTR promoter in the cell nucleus. The present studies show that cells adhere to both synthetic and recombinant Tat, and, using synthetic peptides, we localize the binding site to a region spanning amino acid residues 49-57 (peptide Tat49-57). Tat49-57 also inhibited cell attachment to solid phase full-length Tat peptide and to recombinant Tat protein… CONTINUE READING