Identification of a novel binding partners for tumor suppressor PTEN by a yeast two-hybrid approach.

@article{Gorbenko2004IdentificationOA,
  title={Identification of a novel binding partners for tumor suppressor PTEN by a yeast two-hybrid approach.},
  author={Olena N Gorbenko and Vitaliy S. Kuznetsov and Olexandr Kukharenko and Alexandr Zhyvoloup and Ganna G Panasyuk and Ivan O Nemazanyy and Valeriy Filonenko and Ivan Gout},
  journal={Eksperimental'naia onkologiia},
  year={2004},
  volume={26 1},
  pages={15-9}
}
AIM To identify novel PTEN-binding partners. METHODS The technique of yeast two-hybrid screening was used in this study. A panel of bait constructs was created, containing the C-terminal domain of PTEN, full length PTEN, activated and phosphatase-dead mutants. The expression of LexA-fused baits, their nuclear localization and autoactivation potential were tested according to the standard protocol of Duplex A system. CDNA libraries from Colon Cancer, HeLa and Mouse Embryo were screened with… CONTINUE READING