Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis.

@article{Finkbeiner2009IdentificationOA,
  title={Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis.},
  author={Stacy R. Finkbeiner and Yan Li and Susan J Ruone and Christina B Conrardy and Nicole A Gregoricus and Denise Toney and Herbert W Virgin and Larry J. Anderson and J. Vinje and David Wang and Suxiang Tong},
  journal={Journal of virology},
  year={2009},
  volume={83 20},
  pages={
          10836-9
        }
}
The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides… CONTINUE READING

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