Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge

@article{Ito2011IdentificationOA,
  title={Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge},
  author={Tsukasa Ito and Kazumi Yoshiguchi and Herto Dwi Ariesyady and Satoshi Okabe},
  journal={The ISME Journal},
  year={2011},
  volume={5},
  pages={1844-1856}
}
Major acetate-utilizing bacterial and archaeal populations in methanogenic anaerobic digester sludge were identified and quantified by radioisotope- and stable-isotope-based functional analyses, microautoradiography-fluorescence in situ hybridization (MAR-FISH) and stable-isotope probing of 16S rRNA (RNA-SIP) that can directly link 16S rRNA phylogeny with in situ metabolic function. First, MAR-FISH with 14C-acetate indicated the significant utilization of acetate by only two major groups… CONTINUE READING

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