Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.

@article{Futamura2013IdentificationOA,
  title={Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.},
  author={Yushi Futamura and Makoto Kawatani and Makoto Muroi and Harumi Aono and Toshihiko Nogawa and Hiroyuki Osada},
  journal={Chembiochem : a European journal of chemical biology},
  year={2013},
  volume={14 18},
  pages={2456-63}
}
In the course of screening our microbial metabolite fraction library, we identified a novel pyrrolizidinone compound, pyrrolizilactone. In this study, we report the identification and characterization of a molecular target for pyrrolizilactone by using two phenotypic profiling systems. Cell morphology-based profiling analysis using an imaging cytometer (MorphoBase) classified pyrrolizilactone as a proteasome inhibitor. Consistently, proteome-based profiling analysis using 2D difference gel… CONTINUE READING