Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells.

@article{Maekawa1991IdentificationOA,
  title={Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells.},
  author={Takeo Maekawa and R B Leslie and Ryoko Kuriyama},
  journal={European journal of cell biology},
  year={1991},
  volume={54 2},
  pages={255-67}
}
A mitosis-specific centrosomal component was studied with a human autoantibody, SP-H, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kDa in various lines of cultured cells. Early mitotic PtK1 cells treated with 10 micrograms/ml taxol contained short bundles of parallel microtubules around the nuclei and cell periphery. At the time of nuclear envelope breakdown, the nuclear staining by SP-H disappeared, and the… CONTINUE READING

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