Identification of a membrane protein and a truncated LysR-type regulator associated with the toluene degradation pathway in Pseudomonas putida F1

@article{Wang2004IdentificationOA,
  title={Identification of a membrane protein and a truncated LysR-type regulator associated with the toluene degradation pathway in Pseudomonas putida F1},
  author={Ying Wang and Merriann Rawlings and David Templeton Gibson and Diane Labbé and Hélène Bergeron and Roland Brousseau and Peter C.K. Lau},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={246},
  pages={570-579}
}
A 3 kb DNA region upstream of the toluene degradation (tod) genes, todFC1C2BADEGIH, in Pseudomonas putida F1 (PpF1) was sequenced. Two divergently arranged open reading frames, todR and todX, were identified. A toluene-inducible promoter was localized in front of todX, and the transcription start point was mapped. This promoter is probably responsible for the expression of all tod structural genes. TodX was found to be a membrane protein. Its predicted amino acid sequence (453 residues; Mr… 
Characterization of a new solvent-responsive gene locus in Pseudomonas putida F1 and its functionalization as a versatile biosensor.
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A whole-cell bioluminescent biosensor, PpF1G4, which contains a chromosomally based sep-lux transcriptional fusion and represents a second-generation biosensor that is not based on a catabolic promoter but is nonetheless inducible by aromatic pollutants and moreover functional under nutrient-rich conditions.
Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida
TLDR
It is shown that transcription from P (tmoX) and P(todX) can be mediated by TmoS-TmoT or TodS-todT, in the presence of toluene, revealing cross-regulation between these two catabolic pathways.
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TLDR
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Expansion of growth substrate range in Pseudomonas putida F1 by mutations in both cymR and todS, which recruit a ring-fission hydrolase CmtE and induce the tod catabolic operon, respectively.
TLDR
This study showed that CmtE has a broad range of substrates and can hydrolyse meta-cleavage products formed from biphenyl and other new growth substrates via the toluene degradation pathway, and showed that the TodS sensor was altered to recognize these substrates.
P . putida F 1 except for a single base difference in the operator – promoter region of the cmt operon
TLDR
Results indicate that cmtE is not effectively repressed by CymR in strain CE2010, leading to low constitutive expression and, therefore, low growth on biphenyl.
A β-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator
TLDR
Structural and topology analysis indicated TcpY is an outer membrane protein that facilitates the passing of polychlorophenols across the outer membrane of C. necator, and other COG4313 proteins are possibly outer membrane transporters of hydrophobic aromatic compounds.
Isopropylbenzene catabolic pathway in Pseudomonas putida RE204: nucleotide sequence analysis of the ipb operon and neighboring DNA from pRE4
Pseudomonas putida RE204 employs a set of plasmid-specified enzymes in the catabolism of isopropylbenzene (cumene) and related alkylbenzenes. A 21,768 bp segment of the plasmid pRE4, whose sequence
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