Identification of a major determinant of mouse neurovirulence of dengue virus type 2 using stably cloned genomic-length cDNA.

@article{Gualano1998IdentificationOA,
  title={Identification of a major determinant of mouse neurovirulence of dengue virus type 2 using stably cloned genomic-length cDNA.},
  author={Rosa C. Gualano and Melinda J. Pryor and M R Cauchi and Peter J. Wright and Andrew D. Davidson},
  journal={The Journal of general virology},
  year={1998},
  volume={79 ( Pt 3)},
  pages={437-46}
}
A genomic-length cDNA clone corresponding to the RNA of dengue virus type 2 (DEN-2) New Guinea C strain (NGC) was constructed in a low copy number vector. The cloned cDNA was stably propagated in Escherichia coil and designated pDVWS501. RNA transcripts produced in vitro from the cDNA using T7 RNA polymerase yielded infectious virus (MON501) upon electroporation into BHK-21 cells. When compared with parental NGC virus, MON501 replicated to similar levels in Aedes albopictus C6/36 cells and… CONTINUE READING
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Phylogenetic relationships of dengue-2 viruses

  • J. A. Lewis, Chang, +4 authors D. W. Trent
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