Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product).

@article{Georgopoulos1978IdentificationOA,
  title={Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product).},
  author={Costa P. Georgopoulos and Barbara Hohn},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1978},
  volume={75 1},
  pages={131-5}
}
Mutations in the groE gene of Escherichia coli, which block the correct assembly of the phage lambda head, have been previously described. Many groE mutations exert pleiotropic effects, such as inability to propagate phages T4 and T5 and inability to form colonies at 43 degrees. With the help of the EcoRI and HindIII restrictionenzymes and the appropriate phage vectors, we have constructed two lambda transducing phages, called W3 and H18, that carry the groE+ bacterial gene. Upon lysogenization… CONTINUE READING