Identification of a host 14-3-3 Protein that Interacts with Xanthomonas effector AvrRxv.

  title={Identification of a host 14-3-3 Protein that Interacts with Xanthomonas effector AvrRxv.},
  author={Maureen C Whalen and Todd Richter and Kseniya Zakhareyvich and Masayasu Yoshikawa and Dana Al-Azzeh and Adeshola Adefioye and Greg S. Spicer and Laura L Mendoza and Christine Q Morales and Vicki Klassen and Gina Perez-Baron and Carole S Toebe and Ageliki Tzovolous and Emily Gerstman and Erika E. Evans and Cheryl A. Thompson and Mary J. Lopez and Pamela C. Ronald},
  journal={Physiological and molecular plant pathology},
  volume={72 1-3},
AvrRxv is a member of a family of pathogen effectors present in pathogens of both plant and mammalian species. Xanthomonas campestris pv. vesicatoria strains carrying AvrRxv induce a hypersensitive response (HR) in the tomato cultivar Hawaii 7998. Using a yeast two-hybrid screen, we identified a 14-3-3 protein from tomato that interacts with AvrRxv called AvrRxv Interactor 1 (ARI1). The interaction was confirmed in vitro with affinity chromatography. Using mutagenesis, we identified a 14-3-3… CONTINUE READING
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Short protocols in molecular biology

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