Identification of a heat-modifiable protein of Francisella tularensis and molecular cloning of the encoding gene.

@article{Nano1988IdentificationOA,
  title={Identification of a heat-modifiable protein of Francisella tularensis and molecular cloning of the encoding gene.},
  author={Francis E Nano},
  journal={Microbial pathogenesis},
  year={1988},
  volume={5 2},
  pages={109-19}
}
As an initial step in defining the constituents of the outer surfaces of Francisella tularensis, membrane fractions were prepared, and the immunoreactivity of constituents examined by Western immunoblotting. One protein, thought to be an outer membrane protein, was found to be heat and beta-mercaptoethanol (2-ME)-modifiable and was named FopA. This protein migrates at an apparent molecular weight of 34 kilodaltons (kDa) when cell extracts are solubilized below 80 degrees C, but migrates as a… CONTINUE READING
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