Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy.

@article{Zhou2006IdentificationOA,
  title={Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy.},
  author={Min Zhou and Honglin Xu and Xiaoli Wei and Zhiqiang Ye and Liping Wei and Weimin Gong and Yongqin Wang and Z. -B. Zhu},
  journal={Plant physiology},
  year={2006},
  volume={140 1},
  pages={184-95}
}
5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) is a key enzyme in the shikimate pathway and is targeted by the wide-spectrum herbicide glyphosate. Here, we describe the use of a selection system based on directed evolution to select glyphosate-resistant mutants of EPSPS. Using this system, the rice (Oryza sativa) EPSPS gene, mutagenized by Error-Prone polymerase chain reaction, was introduced into an EPSPS-deficient Escherichia coli strain, AB2829, and transformants were selected on… CONTINUE READING
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