Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment.

Abstract

Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.

DOI: 10.1093/infdis/jit076

Cite this paper

@article{Zhang2013IdentificationOA, title={Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment.}, author={Xinglin Zhang and Janetta Top and Mark de Been and Damien Bierschenk and Malbert Rogers and Masja Leendertse and Marc J M Bonten and Tom van der Poll and Rob J L Willems and Willem van Schaik}, journal={The Journal of infectious diseases}, year={2013}, volume={207 11}, pages={1780-6} }