Identification of a functionally important conformation-sensitive region of the secretory Na+-K+-2Cl- cotransporter (NKCC1).

@article{Dehaye2003IdentificationOA,
  title={Identification of a functionally important conformation-sensitive region of the secretory Na+-K+-2Cl- cotransporter (NKCC1).},
  author={Jean Paul Dehaye and Akos Nagy and Anita Premkumar and Raymond J Turner},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 14},
  pages={
          11811-7
        }
}
The secretory Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) is a member of a small gene family of electroneutral salt transporters that play essential roles in salt and water homeostasis in many mammalian tissues. We have identified a highly conserved residue (Ala-483) in the sixth membrane-spanning segment of rat NKCC1 that when mutated to cysteine renders the transporter sensitive to inhibition by the sulfhydryl reagents 2-aminoethyl methanethiosulfonate (MTSEA) and 2-(trimethylammonium)ethyl… CONTINUE READING
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