Corpus ID: 38538153

Identification of a functional domain within the essential core of histone H3 that is required for telomeric and HM silencing in Saccharomyces cerevisiae.

@article{Thompson2003IdentificationOA,
  title={Identification of a functional domain within the essential core of histone H3 that is required for telomeric and HM silencing in Saccharomyces cerevisiae.},
  author={Jeffrey S. Thompson and M. L. Snow and Summer Giles and Leslie E McPherson and M. Grunstein},
  journal={Genetics},
  year={2003},
  volume={163 1},
  pages={
          447-52
        }
}
Fourteen novel single-amino-acid substitution mutations in histone H3 that disrupt telomeric silencing in Saccharomyces cerevisiae were identified, 10 of which are clustered within the alpha1 helix and L1 loop of the essential histone fold. Several of these mutations cause derepression of silent mating locus HML, and an additional subset cause partial loss of basal repression at the GAL1 promoter. Our results identify a new domain within the essential core of histone H3 that is required for… Expand
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References

SHOWING 1-10 OF 39 REFERENCES
Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.
TLDR
The results support an active role for histone H4 in the silencing of gene expression at this locus and a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML. Expand
COMPASS, a Histone H3 (Lysine 4) Methyltransferase Required for Telomeric Silencing of Gene Expression*
TLDR
It is reported that COMPASS catalyzes methylation of the fourth lysine of histone H3 in vitro, which is required for hist one H3 methylation in vivo and for transcriptional silencing of a gene located near a chromosome telomere. Expand
Identification of a non‐basic domain in the histone H4 N‐terminus required for repression of the yeast silent mating loci.
TLDR
It is proposed that the basic and non‐basic domains together form a DNA (or protein) induced amphipathic alpha‐helix required in the formation of a repressive chromatin structure. Expand
Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast
TLDR
It is reported that histone H3 is required for full repression at yeast telomeres and at partially disabled silent mating loci, and that the acetylatable lysine residues of H3 play an important role in silencing. Expand
Dot1p Modulates Silencing in Yeast by Methylation of the Nucleosome Core
DOT1 was originally identified as a gene affecting telomeric silencing in S. cerevisiae. We now find that Dot1p methylates histone H3 on lysine 79, which maps to the top and bottom of the nucleosomeExpand
Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast
TLDR
Surprisingly, removal of the H4 N terminus also derepresses the silent mating type loci, HML alpha and HMRa, disrupting mating, and this activation is specific since other regulated genes are repressed and induced normally in these cells. Expand
Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.
TLDR
It is reported that lysine (Lys) 79 of histone H3, which resides in the globular domain, is methylated in eukaryotic organisms and indicates that histone modifications in the core globulardomain have important biological functions. Expand
Genetic evidence for an interaction between SIR3 and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae.
TLDR
It is shown here that four positions of histone H4 (N-terminal residues 16, 17, 18, and 19) are crucial to silencing and provides a link between one of the SIR proteins and a component of chromatin. Expand
Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: A molecular model for the formation of heterochromatin in yeast
TLDR
It is shown that the SIR3 and SIR4 proteins interact with specific silencing domains of the H3 and H4 N-termini in vitro, which proposes a model for heterochromatin-mediated transcriptional silencing in yeast, which may serve as a paradigm for other eukaryotic organisms as well. Expand
Silencers and domains of generalized repression.
TLDR
Examination of endonuclease-sensitive sites provided evidence that neither the integrity of the chromosomal duplex nor covalent linkage of the silencers to HMR was important for maintenance of the silenced structure in vitro. Expand
...
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