Identification of a developmentally regulated phase of postselection expansion driven by thymic epithelium.

@article{Hare1998IdentificationOA,
  title={Identification of a developmentally regulated phase of postselection expansion driven by thymic epithelium.},
  author={Katherine J. Hare and Robert W. Wilkinson and Eric J. Jenkinson and Graham Anderson},
  journal={Journal of immunology},
  year={1998},
  volume={160 8},
  pages={3666-72}
}
To investigate events following the initiation of positive selection, we have used reaggregate organ cultures to follow the maturation of purified CD4+8+69+ thymocytes; these thymocytes represent a subpopulation of thymocytes which have already received positive selection signals. Using a dilution analysis of an FITC-based membrane-binding dye, 5-(and -6)-carboxyfluorescein diacetate succinimidyl ester, to allow a quantitative measure of proliferation, we show that while newly selected CD4+ and… CONTINUE READING