Identification of a cleavage site directing the immunochemical detection of molecular abnormalities in type IIA von Willebrand factor.

@article{Dent1990IdentificationOA,
  title={Identification of a cleavage site directing the immunochemical detection of molecular abnormalities in type IIA von Willebrand factor.},
  author={Judith A. Dent and Scott D. Berkowitz and Jerry Ware and Carol K. Kasper and Zaverio M. Ruggeri},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 16},
  pages={6306-10}
}
Proteolytic cleavage of the von Willebrand factor subunit may be important for processing and/or function of the molecule and is altered in certain subtypes of von Willebrand disease. It results in the generation of two main fragments with apparent molecular masses of 140 kDa and 176 kDa from the 225-kDa subunit. We have now obtained chemical evidence to locate the protease-sensitive bond between residues Tyr-842 and Met-843, a site that appears to reflect the specificity of calcium-dependent… CONTINUE READING