Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 Tat transactivator.

@article{Kamine1996IdentificationOA,
  title={Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 Tat transactivator.},
  author={James Kamine and Brindha Elangovan and Thirugnana Subramanian and David Coleman and G. Chinnadurai},
  journal={Virology},
  year={1996},
  volume={216 2},
  pages={
          357-66
        }
}
The Tat protein of the human immunodeficiency virus (HIV) is a powerful activator of HIV gene expression. Genetic and biochemical evidence suggests that one or more cellular cofactors may be important for Tat activity. We have used two-hybrid interactive cloning in yeast to identify a partial cDNA clone (clone 10) from a human B-lymphoblastoid library that specifically interacts with the N-terminal 31 amino acids of HIV-1 Tat which contains the essential cysteine-rich portion of the Tat… 
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