Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.

@article{Ohyama2008IdentificationOA,
  title={Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis.},
  author={Kentaro Ohyama and Mari Ogawa and Yoshikatsu Matsubayashi},
  journal={The Plant journal : for cell and molecular biology},
  year={2008},
  volume={55 1},
  pages={152-60}
}
Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first… CONTINUE READING
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