Identification of a Stat Gene That Functions in Drosophila Development

  title={Identification of a Stat Gene That Functions in Drosophila Development},
  author={Riqiang Yan and Stephen Small and Claude Desplan and Charles R. Dearolf and James E. Darnell},

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Transcriptional Regulation of the Drosophila rafProto-oncogene by Drosophila STAT during Development and in Immune Response*

It is proposed that D-STAT can participate in regulation of the mitogen-activated protein kinase cascade through D-raf gene activation through the D- STAT-binding site.

A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway.

The GMR-upd line represents a system in which the proliferation and differentiation of eye precursor cells are separable and is demonstrated that the enlarged-eye phenotype is a result of an increase in cell number, and not cell volume, and arises from additional mitoses in larval eye discs.

The Drosophila STAT protein, stat92E, regulates follicle cell differentiation during oogenesis.

It is concluded that stat92E is necessary for the early differentiation of follicle cells and for proper germ line cell encapsulation during Drosophila oogenesis.

Identification of Drosophila Genes Modulating Janus Kinase/Signal Transducer and Activator of Transcription Signal Transduction

A dosage-sensitive dominant eye overgrowth phenotype caused by ectopic activation of the JAK/STAT pathway is used to screen 2267 independent, newly generated mutagenic P-element insertions to assemble a collection of genes whose products represent novel components and regulators of this important signal transduction cascade.

The roles of the Drosophila JAK/STAT pathway

The roles of STAT and its associated signaling pathway are reviewed during both embryonic and adult stages of Drosophila development and future prospects for the identification and characterization of novel pathway components and targets are discussed.



Stripe-specific regulation of pair-rule genes by hopscotch, a putative Jak family tyrosine kinase in Drosophila.

The hop gene encodes a putative nonreceptor tyrosine kinase of the Janus kinase family, based on an internal duplication of the catalytic domain, which provides the first evidence for stripe-specific regulation of pair-rule genes by a tyrosin kinase.

Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects.

It is concluded that hopTum‐l encodes a hyperactive Hop kinase and that overactivity of Hop in lymph glands causes malignant neoplasia of Drosophila blood cells.

Characterization and localization of the even‐skipped protein of Drosophila.

The pair‐rule gene even‐skipped of Drosophila is isolated and it is shown that the eve protein is distributed in a series of seven transverse stripes at the cellular blastoderm stage, and is localized primarily within the nuclear regions of those embryonic cells that express the gene.

Transcriptional regulation of a pair-rule stripe in Drosophila.

These experiments suggest that repression involves a competition or short-range quenching mechanism, whereby the binding of gt and Kr interferes with the binding or activity of bcd and hb activators at overlapping or neighboring sites within the eve stripe 2 promoter element.

The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.

Using a combination of promoter fusion-P-transformation assays, and in vitro DNA-binding assays coupled with site-directed mutagenesis, a direct link between dl-binding sites and twi expression in the early embryo is established.

Sequence-specific DNA-binding activities of the gap proteins encoded by hunchback and Krüppel in Drosophila

It is reported that both of the proteins encoded by hunchback and Krüppel possess sequence-specific DNA-binding activities, which indicates that they might regulate gene expression at the level of transcription.

Isolation of the Drosophila segmentation gene runt and analysis of its expression during embryogenesis.

The runt gene encoded a 2.6-kb poly(A)+ RNA that underwent a series of dynamic changes in its spatial and temporal patterns of accumulation during embryogenesis and was most abundant at the blastoderm stage when it showed the seven stripes of expression characteristic of other Drosophila pair-rule genes.

An amino acid substitution in the Drosophila hopTum‐l Jak kinase causes leukemia‐like hematopoietic defects.

It is demonstrated that the hopTum‐l hematopoietic phenotype is caused by a single amino acid substitution of glycine to glutamic acid at residue 341, and the results indicate that a mutant Jak kinase can cause leukemia‐like abnormalities.

Regulation of even‐skipped stripe 2 in the Drosophila embryo.

It is proposed that the clustering of activator and repressor binding sites in the stripe 2 element is required to bring these weakly interacting regulatory factors into close apposition so that they can function both cooperatively and synergistically to control transcription.

Multiple upstream regulatory elements control the expression of the Drosophila white gene.

It is suggested that regulatory elements introduced into the germ line of white mutant flies can act as enhancer‐like elements to regulate the activity of the white promoter and, at least in the case of the zeste regulatory site, that they can act also in ‘trans’ on a white promoter locked in close physical proximity by homologous chromosome pairing.