Identification of a Regulated Pathway for Nuclear Pre-mRNA Turnover


We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA degradation resulted in increased levels of pre-mRNAs and spliced mRNAs. When splicing was inhibited by mutation of a splicing factor, inhibition of turnover resulted in 20- to 50-fold accumulation of pre-mRNAs, accompanied by increased mRNA production. Splicing of a reporter construct with a 3' splice site mutation was also increased on inhibition of turnover, showing competition between degradation and splicing. We propose that nuclear pre-mRNA turnover represents a novel step in the regulation of gene expression.

DOI: 10.1016/S0092-8674(00)00065-9

Extracted Key Phrases

7 Figures and Tables

Citations per Year

1,114 Citations

Semantic Scholar estimates that this publication has 1,114 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{BousquetAntonelli2000IdentificationOA, title={Identification of a Regulated Pathway for Nuclear Pre-mRNA Turnover}, author={C{\'e}cile Bousquet-Antonelli and Carlo Presutti and David Tollervey}, journal={Cell}, year={2000}, volume={102}, pages={765-775} }