Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB

@article{Costa2014IdentificationOA,
  title={Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB},
  author={S{\'e}rgio P. Fernandes da Costa and C. Savva and M. Bokori-Brown and C. Naylor and D. Moss and A. Basak and R. Titball},
  journal={Toxins},
  year={2014},
  volume={6},
  pages={1049 - 1061}
}
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood… Expand
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The structure of the monomeric form of NetB was determined, several residues that are critical for NetB-induced cell lysis were identified, and electrophysiological experiments with planar lipid bilayers revealed that NetB formed pores with much larger single-channel conductance than alpha-hemolysin. Expand
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