Identification of a Gs activator region of the beta 2-adrenergic receptor that is autoregulated via protein kinase A-dependent phosphorylation.

@article{Okamoto1991IdentificationOA,
  title={Identification of a Gs activator region of the beta 2-adrenergic receptor that is autoregulated via protein kinase A-dependent phosphorylation.},
  author={Tomomi Okamoto and Yoshihiro Murayama and Yasunori Hayashi and Maiko Inagaki and Etsuro Ogata and Ikuo Nishimoto},
  journal={Cell},
  year={1991},
  volume={67 4},
  pages={723-30}
}
We have localized a G protein activator region of the human beta 2-adrenergic receptor to region beta III-2 (from Arg259 to Lys273). The synthetic beta III-2, corresponding to the C-terminal end of the third cytoplasmic loop, activates Gs at nanomolar concentrations and weakly activates Gi. beta III-2 activates adenylyl cyclase at nanomolar concentrations in wild-type S49 lymphoma membranes, but not in membranes of unc mutant S49 cells, in which Gs is uncoupled from beta-adrenergic stimulation… CONTINUE READING

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