Identification of a Flavin:NADH Oxidoreductase Involved in the Biosynthesis of Actinorhodin

@article{Kendrew1995IdentificationOA,
  title={Identification of a Flavin:NADH Oxidoreductase Involved in the Biosynthesis of Actinorhodin},
  author={S. Kendrew and S. Harding and D. Hopwood and E. Marsh},
  journal={The Journal of Biological Chemistry},
  year={1995},
  volume={270},
  pages={17339 - 17343}
}
The biosynthesis of the polyketide antibiotic actinorhodin by Streptomyces coelicolor involves the oxidative dimerization and hydroxylation of a precursor, most likely dihydrokalafungin, as the final steps in its formation. Mutations in the actVB gene block these last steps, and the mutants secrete kalafungin as a shunt product. To investigate the role of the actVB gene in these transformations, we have overexpressed the gene in Escherichia coli and purified and characterized the recombinant… Expand
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  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 1997
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TLDR
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