Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae.

@article{Zulty1995IdentificationOA,
  title={Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae.},
  author={James J. Zulty and Gerard J. Barcak},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 8},
  pages={
          3616-20
        }
}
DNA sequencing, RNA mapping, and protein expression experiments revealed the presence of a gene, tfoX+, encoding a 24.9-kDa polypeptide, that is transcribed divergently from a common promoter region with the Haemophilus influenzae rec-1+ gene. H. influenzae strains mutant for tfoX failed to bind transforming DNA and were transformation deficient. Primer extension experiments utilizing in vivo total RNA from precompetent and competent H. influenzae cells demonstrated that transcription of tfoX… CONTINUE READING

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