Identification of a DNA fragment encoding a putative membrane transporter protein expressed in Sporothrix schenckii

@article{Sugita1998IdentificationOA,
  title={Identification of a DNA fragment encoding a putative membrane transporter protein expressed in Sporothrix schenckii},
  author={Yasuyuki Sugita and Jun Fukushima and Mari Murakami and Ikuyo Takahashi and Hiroshi Nakajima},
  journal={Mycopathologia},
  year={1998},
  volume={141},
  pages={101-104}
}
We have isolated two DNA fragments from Sporothrix schenckii. These fragments were nonspecifically amplified from the whole-cell DNA using polymerase chain reaction (PCR) primers originally designed in the gene encoding heat shock protein 70 of Mycobacterium leprae. Reverse transcription PCR demonstrated that the smaller (sp-2) fragment was expressed, and a… CONTINUE READING