Identification of a D8S1179 primer binding site mutation and the validation of a primer designed to recover null alleles.

@article{Leibelt2003IdentificationOA,
  title={Identification of a D8S1179 primer binding site mutation and the validation of a primer designed to recover null alleles.},
  author={Craig S. Leibelt and Bruce Budowle and Patrick J. Collins and Yasser Daoudi and Tamyra R Moretti and Gary Nunn and Dennis J. Reeder and Rhonda K. Roby},
  journal={Forensic science international},
  year={2003},
  volume={133 3},
  pages={220-7}
}
A population study of Chamorros and Filipinos using short tandem repeat (STR) loci amplified with the AmpFlSTR Profiler Plus PCR amplification kit demonstrated an excess of observed homozygosity at the D8S1179 locus. Use of a different set of D8S1179 primers to type the same samples did not demonstrate an excess of homozygosity and showed discordant genotypes at the D8S1179 locus. A single point mutation, G-to-A transition, 16 nucleotides from the 3' end of the reverse primer, was identified to… CONTINUE READING

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