Identification of a Chlamydomonas plastidial 2‐lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content

@inproceedings{Yamaoka2016IdentificationOA,
  title={Identification of a Chlamydomonas plastidial 2‐lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content},
  author={Yasuyo Yamaoka and Dorine Achard and Sunghoon Jang and Bertrand L{\'e}geret and Shogo Kamisuki and Donghwi Ko and Miriam Schulz‐Raffelt and Yeongho Kim and Won‐Yong Song and Ikuo Nishida and Yonghua Li‐Beisson and Youngsook Lee},
  booktitle={Plant biotechnology journal},
  year={2016}
}
Despite a strong interest in microalgal oil production, our understanding of the biosynthetic pathways that produce algal lipids and the genes involved in the biosynthetic processes remains incomplete. Here, we report that Chlamydomonas reinhardtii Cre09.g398289 encodes a plastid-targeted 2-lysophosphatidic acid acyltransferase (CrLPAAT1) that acylates the sn-2 position of a 2-lysophosphatidic acid to form phosphatidic acid, the first common precursor of membrane and storage lipids. In vitro… CONTINUE READING
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